Projects / Research Experience

Foster Lab

Cardiac physiology, mitochondrial assays & translational wet lab methods

In the Foster Lab, I support OGT/O-GlcNAc heart projects that connect cardiac stress responses to protein quality control, mitochondrial function, and ischemia/reperfusion injury. My work includes Langendorff preparation, western blot verification, immunoprecipitation support, method documentation, and manuscript or abstract preparation.

Rohit Satish in the Foster Lab wet-lab workspace
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My RoleUndergraduate Researcher
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Location / DatesFoster Lab, Johns Hopkins University
May 2023 – Present
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OutputsWestern blot validation, abstract support, method documentation, and manuscript figures
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Skills LearnedLangendorff perfusion, western blotting, mitochondrial analysis, experimental documentation

Research Overview

My current work sits between two related OGT/O-GlcNAc cardiac projects: an acute ischemic stress-granule manuscript and a broader abstract on how cardiomyocyte OGT loss reshapes mitochondrial and proteostasis networks.

  • Documenting Langendorff heart perfusion and beating-heart setup
  • Checking western blot readouts for OGT, O-GlcNAc, total protein, and selected validation targets
  • Supporting immunoprecipitation workflows used to enrich candidate proteins like G3BP1
  • Organizing snapshots, manuscript figures, abstract files, and presentation material

Researcher Snapshot

Lower ventricular section from a TMG-treated mouse heart after Langendorff perfusion. The left panel shows the cross-section image; the right panel shows the classification outline used to separate live from dead tissue.
Comparison figure from Foster Lab resources

What I Do

Langendorff ProcedureHelp document the ex vivo perfused-heart setup used to test ischemia/reperfusion in intact tissue.
Western Blot ChecksVerify whether OGT/O-GlcNAc and selected validation targets changed before results are interpreted.
Immunoprecipitation SupportSupport protein-enrichment workflows that isolate G3BP1 or candidate targets before blotting or mass spectrometry.
Presentation PrepKeep snapshots, abstract text, figures, and manuscript material organized for poster or paper updates.

Western Blot Output

Example western blot and quantification output from the Swimm supplemental figures, used to verify O-GlcNAc/OGA shifts against total-protein loading.

Western blot validation panels from page 53 of the Swimm manuscript

Research Outputs

Acute OGT/O-GlcNAc injury project connecting G3BP1-linked stress granules, Langendorff ischemia/reperfusion, and cardioprotection.

Chronic OGT-loss abstract focused on mitochondrial decline, proteostasis/HSP responses, remodeling signatures, and western blot validation.